@dlwagnerlabs.bsky.social - DL Wagner Genome Engineering Labs

09 Sep 2026 09:40 +0000

#BEKI is finally published in Molecular Therapy! Viktor Glaser's story outlines a non-viral 'one-shot' approach using normal base-editor enzymes to simultaneously integrate CAR transgenes and knock-out up to 10 genes. Link: https://www.sciencedirect.com/science/article/pii/S1525001626006027

05 Oct 2025 20:51 +0000

Our team is on their way to the ESGCT conference 2025! Excited for this upcoming week full of science, talks, and inspiring discussions with the cell & gene therapy community! Starting with the HDR workshop - talks by Isabell (‘one-pot’ PASTA) & Viktor (BEKI) + posters by Ana, Lily, Jonas and Laura

18 Sep 2025 00:49 +0000

Lab History pt. 6 (2025) For non-viral CAR-T cell generation with Cas12a, what is BETTER THAN DOUBLE-STRANDED DNA ? https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(25)00122-2

18 Sep 2025 00:38 +0000

Lab History pt. 5 (2025) What is the best Knock-in strategy to make CAR-Tregs?? our answer is published in Molecular Therapy 🔗 https://www.cell.com/molecular-therapy-family/molecular-therapy/fulltext/S1525-0016(25)00083-8

18 Sep 2025 00:29 +0000

Lab History pt. 4 (2024) What could be BETTER THAN TRAC KNOCK-IN ?? CD3Z-CAR KI story, published in Blood https://pmc.ncbi.nlm.nih.gov/articles/PMC11196866/

18 Sep 2025 00:23 +0000

Lab History pt. 3 (2023) Ultra-depletion of TCR+ cells from allogeneic CAR T cells to prevent GvHD.... LOG-FOLD BETTER THAN MACS!!! HOW? => Addition of short-lived TCR-specific CAR NK-92 cells during expansion. Published in Blood Advances 🔗 https://ashpublications.org/bloodadvances/article/7/15/4124/495855/CAR-NK-92-cell-mediated-depletion-of-residual-TCR

18 Sep 2025 00:13 +0000

Lab History pt. 2 (2023) Single-step, complex non-viral CAR-T engineering: Cas12a for TRAC KI + nCas9 base editors for multiplex-KO. => Using orthogonal CRISPR systems for KI and KO ↓ translocations to <0.1%. Published in Genome Biology. 🔗 https://link.springer.com/article/10.1186/s13059-023-02928-7

17 Sep 2025 23:51 +0000

Lab History pt. 1 2022 – 🚨 Our first paper is out! 🚨 virus-free CAR knock-in to TRAC locus of primary human T cells with CRISPR-HDR. ➡️ >50% TRAC CD19-CAR T cells through pharmacological modulation 🧬 A key step toward scalable, non-viral CAR-T cell therapies. DOI: 10.1016/j.omtm.2022.03.018