🧠 Neurologist 🇯🇵 | Postdoc @WernigLab @Stanford 🧫 iPSC-derived models for studying neurodegeneration 🔬 Former @brainresilience.bsky.social | Current @hdfcures.bsky.social Fellowhttps://bsky.app/profile/tuenaka.bsky.socialhttps://bsky.app/profile/tuenaka.bsky.social/post/3mfmpqc4sqc2cHave you ever cried in the tissue culture room because your #iPSC transgene got silenced after differentiation? I have. Many, many times. So we built TK4, a PiggyBac vector designed to resist silencing. Now available on @addgene.bsky.social: #239047 / #239046 Would love to hear your thoughts.24 Feb 2026 17:43 +0000at://did:plc:pvej5kujyfh3gnvqxjpwtpha/app.bsky.feed.post/3mfmpqc4sqc2chttps://bsky.app/profile/tuenaka.bsky.social/post/3mfabdxrs3s22Losing sleep over #iPSC transgene silencing after differentiation? Same here. Bright in iPSCs. Gone after differentiation. Our paper in @cp-cellstemcell.bsky.social maps what actually keeps expression on👇 https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(26)00030-5?rss=yes&utm_medium=twitter&utm_source=dlvr.it 🔗 Free access (50 days): https://www.sciencedirect.com/science/authShare/S1934590926000305/20260213T153300Z/1?md5=4f98f223c9a271b3edd4ead8ed0674e7&dgcid=coauthor19 Feb 2026 18:54 +0000at://did:plc:pvej5kujyfh3gnvqxjpwtpha/app.bsky.feed.post/3mfabdxrs3s22https://bsky.app/profile/tuenaka.bsky.social/post/3merekqmp2s2oThrilled to see our paper out in @cp-cellstemcell.bsky.social! [contains quote post or other embedded content]13 Feb 2026 20:41 +0000at://did:plc:pvej5kujyfh3gnvqxjpwtpha/app.bsky.feed.post/3merekqmp2s2ohttps://bsky.app/profile/tuenaka.bsky.social/post/3meranbdwgs2fOur paper on preventing transgene silencing is now online in @cp-cellstemcell.bsky.social! 🔗 Free access (50 days): https://www.sciencedirect.com/science/authShare/S1934590926000305/20260213T153300Z/1?md5=4f98f223c9a271b3edd4ead8ed0674e7&dgcid=coauthor13 Feb 2026 19:31 +0000at://did:plc:pvej5kujyfh3gnvqxjpwtpha/app.bsky.feed.post/3meranbdwgs2fhttps://bsky.app/profile/tuenaka.bsky.social/post/3lmufstv7zk2kLosing sleep over #iPSC transgene silencing after differentiation? Same here — too many times😭 So we built a PiggyBac construct that resists silencing. Expression stays ON, even after differentiation. Our #preprint is out👇 🔗 https://www.biorxiv.org/content/10.1101/2025.04.07.647695v1 Supported by @cziscience.bsky.social15 Apr 2025 15:37 +0000at://did:plc:pvej5kujyfh3gnvqxjpwtpha/app.bsky.feed.post/3lmufstv7zk2k