https://bsky.app/profile/alonsostepanovalab.bsky.socialhttps://bsky.app/profile/alonsostepanovalab.bsky.social/post/3lqct6katik24Mario's vitamin C study uncovers an interesting link between this essential metabolite and the regulation of local auxin biosynthesis https://www.biorxiv.org/content/10.1101/2025.05.15.654287v129 May 2025 13:29 +0000at://did:plc:tha52paszwjcpdcksilk6d5a/app.bsky.feed.post/3lqct6katik24https://bsky.app/profile/alonsostepanovalab.bsky.social/post/3lqcszz7f4s24Development of this new genetic tool was sparked by a spontaneous discussion at a scientific conference. One two-year NSF EAGER grant to jump-start the work and 5 more years of "side projects" by 18 talented scientists made EBSn possible. Good science takes time! https://www.biorxiv.org/content/10.1101/2025.05.23.655144v129 May 2025 13:26 +0000at://did:plc:tha52paszwjcpdcksilk6d5a/app.bsky.feed.post/3lqcszz7f4s24https://bsky.app/profile/alonsostepanovalab.bsky.social/post/3llojkpxsik2rKatie Vollen's PhD prelim exam writeup transformed into a nice review article on large DNA manipulation: https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.70099 Way to go, Katie!31 Mar 2025 14:03 +0000at://did:plc:tha52paszwjcpdcksilk6d5a/app.bsky.feed.post/3llojkpxsik2rhttps://bsky.app/profile/alonsostepanovalab.bsky.social/post/3ljsos3qh622wIf you're struggling to assemble large DNA constructs, check out Chengsong's DASH system, which combines Golden Gate cloning, recombineering, and site-specific recombinases to build multigene constructs of ~100kb. https://www.biorxiv.org/content/10.1101/2025.03.04.641457v107 Mar 2025 18:57 +0000at://did:plc:tha52paszwjcpdcksilk6d5a/app.bsky.feed.post/3ljsos3qh622w